Evaluation of Relationship between Single-nucleotide Polymorphism in TNF-gene Promoter and Susceptibility to Atherosclerosis in Fatemeh Zahra Hospital

Background: Tumor necrosis factor alpha is a proinflammatory cytokine and  as  polyvalent initial response of inflammatory cells which facilitated through the creation of coronary atherosclerosis . It also seems that the polymorphism and susceptibility to atherosclerosis is related to TNF-α gene promoter. The purpose of this study was to investigate single nucleotide polymorphisms of  TNF-α gene promoter  in two sites-863 and-308 in patients with atherosclerosis who referred to Fatemeh al-Zahra  hospital of Sari city. Materials and Methods: This case-control study  was on 120 patients (with  stenosis  more than 50%) and 120 healthy individuals (with stenosis less than 10%) . Genomic DNA was extracted with Phenol-chloroform  procedure  from  their white blood  cells. Genotypes of these people  and TNF-α gene polymorphisms were analyzed by RFLP-PCR method. Genotype frequencies were determined by using the software SPSS version 22 and the Hardy-Weinberg equilibrium and chi- square analysis. Results: Genotype frequencies of  GA, GG, AA position  - 308 TNF-α gene in patients were respectively 12.5,75,12.5  percent  and in  healthy subjects, respectively 7.5, 21.7, 70.8.Allele A to  G allele increases the risk of the disease 12.716 percent. The genotype frequency of the AC, CC, AA position -863 TNF-α gene in patients were respectively 3.3, 69.2, 27.5 percent and in healthy individuals were 2.5, 11.7, 85.8. Allele A to C allele increases the risk of the disease 16.373 percent. Findings and the results of statistical analysis showed that the risk of atherosclerosis with single nucleotide polymorphisms in the TNF-α gene-863 for C   Conclusion: According to this study,mutations in the promoter region of the gene of TNF-α  could have  possibly increased susceptibility to atherosclerosis disease. Determination of  the genotypes of the individuals in these areas can help identification of  patients with  high risk for the cardiac disease.